The Accuris GenoSep™ instrument utilizes convenient, reusable cartridges to separate nucleic acids and proteins. Each cartridge incorporates a proprietary polymer matrix and a fused silica capillary. During analysis, programmable high voltage is applied through the capillary to generate an electric field that drives the electrophoretic migration of macromolecules through the polymer matrix.
Accuris GenoSep™ Σύστημα Τριχοειδούς Ηλεκτροφόρησης
Σύστημα τριχοειδούς ηλεκτροφόρησης για υψηλής ανάλυσης ανάλυση και χαρακτηρισμό DNA, RNA και πρωτεϊνών, με αυτοματοποιημένη λειτουργία και ευαίσθητη ανίχνευση φθορισμού.
Περιγραφή
GenoSep™ Capillary Electrophoresis System
High-Resolution Analysis for DNA, RNA, Proteins & More
The Accuris GenoSep™ is a compact capillary electrophoresis system designed for high-resolution characterization of nucleic acids and proteins. Combining automated electrophoresis with sensitive fluorescence detection, it provides accurate fragment sizing, quantification, and quality assessment of DNA, RNA, proteins, NGS libraries, and cell-free DNA (cfDNA) samples.
GenoSep Separation Technology
Differences in electrophoretic mobility result in size-based separation, and fluorescently labeled fragments are detected as they pass the optical detection window. User-selectable voltages enable methods to be optimized for different applications and fragment size ranges.
Performance
To ensure exceptional sensitivity and reproducibility, each GenoSep™ cartridge is produced under a rigorous manufacturing process to ensure consistent results from cartridge to cartridge.
Precision high-voltage control with automated instrument operation allw the GenoSep™ system to deliver highly reproducible migration times, enabling accurate fragment sizing and reliable comparison between runs. Figure 2 demonstrates the reproducible results obtained from repeated analyses of the same sample. Consistent migration profiles provide reliable assessment of sample integrity.
The GenoSep™ system also provides exceptional sensitivity, with a limit of detection as low as 2 pg (Fig. 3). This high sensitivity is achieved through LED-induced fluorescence detection, optimized optical design, and a proprietary low-background separation matrix that minimizes background noise and enhances signal-to-noise performance.
Software
The included Windows-based GenoSep™ software allows for instrument control and data analysis. The PC software is quick to install, setup, and begin operation.
Running an assay is simple – load the samples, select the desired method, and click Run. The software automatically controls the instrument, monitors the analysis in real time, and generates electropherograms, virtual gel images, fragment sizing info, and quantitative results (Fig. 4).
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