A&A Biotechnology offers a wide range of enzymes for molecular biology applications, including DNA and RNA processing, bacterial lysis, and protein analysis.
Description
Enzymes
A&A Biotechnology offers a diverse range of high-performance enzymes designed for a variety of applications, from DNA and RNA manipulation to bacterial lysis. Their products are known for precision and reliability, making them ideal for use in molecular research, diagnostics, and biotechnology. Whether you need enzymes for PCR, cloning, sequencing, or protein analysis, A&A provides top-tier options that ensure high-quality results.
Products Include:
🔵 T4 DNA polymerase
Reaction buffer included. Form: solution, concentration 3 U/µl.
➤ Description
T4 DNA Polymerase is a template-dependent polymerase that catalyzes 5′-3′ synthesis from primed single-stranded DNA. The enzyme also has 3′-5 ‘exonuclease activity. The T4 DNA polymerase has no 5′-3 ‘exonuclease activity.
The 3′-5 ‘exonuclease activity is much higher for ssDNA compared to dsDNA.
T4 DNA polymerase is active in most buffers used in PCR, reverse transcription, ligation buffer and in most buffers for restriction enzymes
🔵 Tornado™ DNAse
Recombined engineered DNAse I with increased processivity. Concentration: 2 U/µl.
➤ Description
Tornado™ DNAse (RNase-free) is an endonuclease that digests ssDNA, dsDNA and DNA in DNA-RNA complexes. The enzyme contains an additional domain responsible for the effective binding of a substrate DNA. The result is a versatile nuclease that possesses remarkably higher affinity towards dsDNA, even in the nM range of the DNA concentration.
➤ Applications
●Removal of contaminating genomic DNA from RNA samples
●Removal DNA matrix post in vitro transcription.
●Study of DNA-protein interactions by DNase I footprinting
●Preventing false positive PCR results in one-step RT-qPCR
🔵 BacBreaker
Bacteria enzyme mix for lysis of Gram-negative and Gram-positive bacteria. Form: solution.
➤ Description
BacBreaker is a unique mixture of enzymes that effectively lyses the cell walls of Gram-negative and Gram-positive bacteria. Enzyme mix activity is synergistic. Using mixture leads to significantly increased yield of bacteria lysis.
🔵 Exonuclease V (RecBCD)
Recombinant enzyme that cleaves linear single-stranded and double-stranded DNA in both 3′ to 5′ and 5′ to 3′ directions. Concentration 10 U/µl.
➤ Description
Exonuclease V is a complex of recombinant RecB, RecC and RecD proteins from E. coli. Enzyme has a helicase and nuclease activity. In the presence of ATP catalyzes exonucleolytic cleavage in both 3′ to 5′ and 5′ to 3′ directions to yield 5′ phosphooligonucleotides.
➤ Advantages
- DNA specific exonuclease that has ssDNA and dsDNA exonuclease activity and ATP-dependent ssDNA endonuclease activity.
- Cleaves ssDNA and dsDNA in both 3′ to 5′ and 5′ to 3′ directions.
- Degrades linear ssDNA and dsDNA leaving intact supercoiled and circular dsDNA.
IDEAL FOR
- DNA/RNA manipulation (e.g., synthesis, degradation)
- Protein analysis (e.g., purification, modification)
- Bacterial lysis (e.g., disrupting cell walls)
- PCR (e.g., amplification)
- Cloning and sequencing (e.g., gene editing, amplification)
- Diagnostics and research (e.g., laboratory testing, molecular studies)
FEATURES
| Feature | Description |
|---|---|
| High Performance | Optimized for efficient reactions in molecular biology. |
| Versatility | Suitable for a variety of applications like DNA/RNA processing, protein analysis, and bacterial lysis. |
| Reliability | Ensures consistent, reproducible results in experiments. |
| Wide Application Range | Ideal for use in research, diagnostics, and biotechnology. |
| Convenience | Easy to use and integrates well into laboratory protocols. |
