A&A Biotechnology offers a comprehensive range of molecular weight markers designed for precise sizing of nucleic acids during electrophoresis. These markers are essential tools in molecular biology laboratories, providing reliable references for DNA and RNA fragment analysis.
Description
Molecular Weight Markers
A&A Biotechnology‘s molecular weight markers are high-quality, ready-to-use tools designed for precise and reliable size estimation of DNA and RNA fragments during electrophoresis. They are essential for accurate analysis in molecular biology research and diagnostics.
These markers offer:
- Wide size range: Available options cover various fragment sizes, from 50 bp up to 8,126 bp, suitable for both small and large nucleic acid analysis.
- High concentration: Typically at 0.1 µg/µl, ensuring clear and well-defined bands for accurate measurements.
- Ready-to-use format: Pre-mixed with loading dye for direct application onto agarose gels, saving time and minimizing errors.
- Sharp and stable bands: Optimized for consistent migration and clear visibility, even for small fragments.
- Compatibility: Ideal for agarose gel electrophoresis, suitable for standard and high-resolution applications.
- Long shelf life: Stable under standard storage conditions, ensuring long-term reliability.
A&A’s molecular weight markers are the perfect reference tools for PCR product analysis, restriction enzyme digestion, and nucleic acid fragment verification.
Products:
IDEAL FOR
✔️ DNA Fragment Size Determination: Used in agarose gel electrophoresis to estimate the size of DNA fragments by comparing their migration with that of the markers.
✔️ DNA Concentration Assessment: By comparing the intensity of DNA bands in the sample to those of the marker, scientists can estimate the DNA concentration.
✔️ PCR Product Analysis: Ensures the accuracy of polymerase chain reaction (PCR) by verifying the size of the amplified DNA fragments.
✔️ Restriction Enzyme Digestion Check: Helps in checking the completeness of DNA digestion by restriction enzymes by comparing the expected fragment sizes.
✔️ Cloning Experiment Validation: Confirms the insertion of the desired DNA fragment into vectors by comparing the sizes of the inserts.
✔️ Protein Analysis: In protein electrophoresis (SDS-PAGE), molecular weight markers (protein ladders) are used to estimate the molecular weight of proteins.
✔️ RNA Analysis: In RNA electrophoresis, molecular weight markers help determine the size of RNA molecules, aiding in the study of gene expression and RNA processing.
FEATURES
| Characteristic | Description |
|---|---|
| Size range | Covers various ranges, such as 50-1,000 bp, 100-3,000 bp, 151-8,126 bp, depending on the marker type. |
| Concentration | Typically 0.1 µg/µl, ensuring sufficient quantity for analysis. |
| Format | Provided in a ready-to-use form, allowing immediate application. |
| Applications | Suitable for agarose gel electrophoresis, used to estimate the size of DNA or RNA fragments. |
