FineTest offers over 5,000 recombinant proteins, expressed in HEK293 and E.Coli systems, for applications such as WB and ELISA, covering common species and research fields, including inflammatory factors, signaling pathways, diagnostic materials, receptors, enzymes, and antigens.
Description
Protein
FineTest Recombinant Protein
FineTest® protein expression platform solves various difficulties in protein expression and expression to meet different applications. Our powerful R & D team is committed to be your best experimental partner and offers customers well-designed protein sequences, the best vector and label, greatly ensuring solubility and activity of proteins.
FineTest® protein services provided over 5,000 recombinant proteins expressed by HEK293 and E.Coli for WB, ELISA application, covering common species(e.g. human) for inflammatory factors, signaling pathways, diagnostic materials, receptor proteins, enzymes, antigens etc. Besides His tag and GST tag, we developed special IF2DI tag with high-efficient solubility which can greatly improve the protein expression. Featured services like protein labeling and conjugation are also available. Also we accept custom protein production.
FineTest® protein expression platform uses multiple purification methods to maintain the protein purity, including affinity purification, ion exchange, hydrophobic chromatography etc. We also have advanced refolding technology for insoluble proteins, and try our best to restore protein conformation as well as keeping its property to natural protein.
Advantages of FineTest® Protein
Soluble Protein
The self-developed pET28a-IF2DI expression vector has the powerful IF2DI tag, which has better soluble expression effect on very hydrophobic proteins. Perfectly replace tags like GST, MBP etc. The smaller molecular weight has a lower effect on the expression of target proteins.
Unlabeled Protein
According to customers’ requirements, IF2DI tag can be removed by Thrombin cleavage site to obtain the unlabeled protein. There is no effect on the protein activity.
Inclusion Body Protein
For insoluble protein aggregates, FineTest uses the unique protein refolding technolog to refold in the suitable buffer system and also obtain the target protein in the form of supernatant. The activity of nearly 100 proteins has been validated.
Custom Recombinant Protein
FineTest can produce any custom protein according to customers’ requirements. Only protein name, Uniprot ID and applications are required.
Publications
Since 2016, FineTest products have been used by global and domestic scientific research institutions, universities and pharmaceutical platforms. The quantity of citations are always rapidly increasing due to the investment on R & D of our products. Currently, over 3,300 citations have proven the stable quality widely accepted by our customers. The total IF is nearly 12,000. The highest and average IF are 46.351 and 3.51 respectively. There is a fast annual increase of citations and IF. Especially some of journals are highly ranked in the discipline.(e.g. Cell Research, Cell, Advanced Materials, Annals of the Rheumatic Diseases, Materials Today, Cellular & Molecular Immunology, Journal of Medical Virology etc.)
IDEAL FOR
FineTest proteins are ideal for research and diagnostic applications such as:
- Western Blot (WB)
- ELISA
- Immunohistochemistry (IHC)
- Flow Cytometry
- Protein Function Assays
They are used in fields such as immunology, oncology, neuroscience, and diagnostic kit development.
FEATURES
| Characteristic | Details |
|---|---|
| Type | Recombinant & Natural Proteins |
| Expression Systems | HEK293, E.Coli |
| Applications | WB, ELISA, IHC, Flow Cytometry, Functional Assays |
| Research Fields | Immunology, Oncology, Neuroscience, Inflammation, Diagnostics |
| Purity | > 90% (SDS-PAGE) |
| Form | Liquid or Lyophilized |
| Species Covered | Human, Mouse, Rat, etc. |
| Example Proteins | Cytokines, Enzymes, Receptors, Antigens |
| Certifications | QC-tested, High Reproducibility |
Example Proteins
| Protein Name | Category | Applications |
|---|---|---|
| IL-6 (Interleukin-6) | Cytokine | Immunology, Inflammation |
| TNF-α (Tumor Necrosis Factor-α) | Cytokine | Inflammation, Oncology |
| EGF (Epidermal Growth Factor) | Growth Factor | Cell Growth, Cancer Research |
| HER2 (Human Epidermal Growth Factor Receptor 2) | Receptor | Oncology, Cancer Research |
| Beta-Amyloid 42 | Neuroscience | Alzheimer’s Research |
| PSA (Prostate-Specific Antigen) | Diagnostic Marker | Prostate Cancer Detection |
